Håkan Håkansson

RESEARCH INTERESTS High-end mass spectrometry, including Fourier transform ion cyclotron resonance (FT-ICR), orbitrap, and ion mobility spectrometry, for biomolecular identification and structural characterization: •Characterization of nucleic acid structure through gas-phase ion-electron reactions, e.g., electron capture dissociation (ECD), negative ion electron capture dissociation (niECD, discovered in the Hakansson laboratory), and electron detachment dissociation (EDD). •Identification and characterization of protein posttranslational modifications by complementary fragmentation techniques such as ECD/niECD, electron transfer dissociation (ETD), free radical initiated peptide sequencing (FRIPS), and infrared multiphoton dissociation (IRMPD) at low (fmol) levels. •Exploration of the gas-phase fragmentation behavior of biomolecules following ion-electron reactions. •Application of ion-electron and ion-photon reactions in cancer glycomics and sulfoproteomics. •Probing covalent intermediates in the catalysis of non-ribosomal peptide synthetases and polyketide synthases. •Targeted proteomics of natural product biosynthetic enzymes. •Mapping macromolecular contact surfaces by solution-phase hydrogen/deuterium exchange (tandem) mass spectrometry.

Faculty Member · Florida State University